Monthly Archives: August 2018

The Last Weeks of Summer

By: Connie Machuca

Greetings from The Wrigley Institute for Environmental Studies! It is currently week 9 of the REU (Research Experience for Undergraduates) program. I cannot believe this summer has gone by so fast. It feels like just yesterday I was getting on the Miss Christi for the first time with all the other REUs, many of them still strangers to me. Coming to Catalina for the first time, I did not know what to expect when we first arrived. I quickly learned that Catalina Island is a warm and welcoming place that supports students by allowing them to pursue their interests while also having fun!

My research partner, Tristan Jordan-Huffman, and I out planting some juvenile kelp into Big Fisherman’s Cove with the help of our mentor Diane Kim.

My research partner, Tristan Jordan-Huffman, and I out planting some juvenile kelp into Big Fisherman’s Cove with the help of our mentor Diane Kim.

My summer research project is a part of a bigger project at Wrigley that is attempting to grow and use kelp as a source of biofuel. This project is funded by The Department of Energy, more specifically the Advanced Research Projects Agency. Both USC’s Wrigley Institute for Environmental Studies and Marine BioEnergy, Inc. are participating in this research. My project relates directly to an aspect of the Kelp Biofuel project called “depth cycling.” Depth cycling would allow kelp to be close to the surface of water, receiving sunlight for photosynthesis during the day, and moving to lower depths at night to receive nutrients.

One of the challenges with depth cycling is that the kelp will be exposed to increased hydrostatic pressure. With every 10 meters reached on descent, pressure is increasing by 1 atm of pressure. My project aims to investigate the effects of increased hydrostatic pressure exposure on the kelp species Macrocystis pyrifera. Some of the questions I wanted to answer are: “Can we maintain the integrity of developed pneumatocysts (air bladders on the kelp) when depth cycling, or will they pop?” And “Can juvenile Macrocystis adapt to higher pressure during development?”

This picture shows the set up for the Catalina Hyperbaric Chamber experiment. Milena and I set up two aquariums and weighed down kelp to expose it to pressure.

This picture shows the set up for the Catalina Hyperbaric Chamber experiment. Milena and I set up two aquariums and weighed down kelp to expose it to pressure.

In order to attempt to answer these questions I used the Catalina Hyperbaric Chamber to expose fully-grown kelp individuals to pressure, as well as a mini pressure chamber to expose juvenile kelp to pressure. This past week I have been wrapping up my experiments and focusing on data analysis. Data analysis has always been a little tough for me, but I’m fortunate to have my mentors Andy and Diane helping me through it all.

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This is a picture of my second experiment involving a mini pressure chamber that would expose juvenile kelp to pressure every day.

Although this past week has been filled with endless data input, graph making on Excel, and scientific poster making, I took a little time off and went for a 2-mile run to Two Harbors! I joined Callie and Kent on their daily run to be a little active during this busy week. Of course, it is not a complete trip to Two Harbors if you don’t get an ice cream sundae while you’re there!

Callie, Kent, and I enjoying some ice cream after our two-mile run.

Callie, Kent, and I enjoying some ice cream after our two-mile run.

One of the last things the REU cohort plans to do together is camping! We will be hiking from Two Harbors to Shark Harbor, a 4.5 mile hike, and camping overnight on the beach! I’m really excited to have some last-minute fun with the whole group before we all go our separate ways for the upcoming semester.

I’m really sad that we only have a week left together on the island. Coming to Catalina with 11 other strangers was a little scary at first, but after spending time together, I realized that we are more than a friend group – we have become a family and I’m so grateful to have had this opportunity with each and every one of them! I can’t say this enough, but this summer was one of the best I’ve ever had and I owe it all to the USC Wrigley Institute for Environmental Studies, The National Science Foundation, and everyone who made this REU experience possible!

New Catalytic Methods for Synthesizing Bioactive Compounds

By: Valeriy Cherepakhin

I am a third year graduate student in Dr. Travis J. Williams’ group in the Chemistry Department at USC. Our research group is concerned with scientific problems in the areas of catalysis, inorganic, and organic chemistry.

We focus on the development of environmentally friendly, sustainable catalytic chemical processes that have economic and ecological advantages over existing ones. More specifically, our ongoing projects are devoted to degradation and recovery of composite materials, conversion of CO2 into renewable fuels, and the development of new synthetic methods to manipulate hydrogen.

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Bioactive compounds are chemicals that possess the capability to affect a living organism in any way. Typically, they are used as drugs to cure, treat or prevent diseases, and therefore play an important role in our survival. Some of these compounds occur naturally in organisms, but many were created artificially through organic synthesis in chemistry labs.

Indole is a compound that gives rise to a range of bioactive molecules. For example, tryptophan is an essential amino acid and a constituent of most proteins; and serotonin is an important neurotransmitter in the nervous, cardiovascular and gastrointestinal systems. The structurally similar hormone melatonin regulates sleep and wakefulness in humans and animals.

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I am happy to have received the 2018 Sonosky Summer Fellowship this summer to conduct research on the catalytic synthesis of new indole derivatives that offer a class of new potentially bioactive compounds. Our current data show that it is possible to enable a reaction to give new compounds (A and B) with a high yield and selectivity. We are planning to investigate the scope of this new reaction to see if it is successful in the presence of other functional groups in substrate molecules.

The results obtained so far are important, since our method conveniently provides access to A and B in just one step, whereas traditional methods require multiple-step synthesis. The work opens the door to new, environmentally benign methods for fine chemical synthesis.

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In conclusion I would like to express my gratitude to a first year graduate student from our lab – Anju Nalikezhathu – who significantly contributed to this project.